Effect of salidroside on regulating phenotypic expressionof human osteoblast-like MG-63 cells through HIF-1α signal passway
10.3969/j.issn.1001-1978.2017.06.018
- VernacularTitle:低氧状态下红景天苷经HIF-1α信号通路调节人成骨样MG-63细胞表型表达的研究
- Author:
Lin QI
;
Chuan WANG
;
Guohua MEI
;
Xiaochang WEN
;
Liqun MAO
;
Xin JIN
;
Ling LI
;
Yue WANG
- Keywords:
salidroside;
hypoxia-inducible factor(HIF)-1α;
osteoblast;
proliferation;
differentiation;
apoptosis;
vascular endothelial growth factor(VEGF)
- From:
Chinese Pharmacological Bulletin
2017;33(6):836-843
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of salidroside on the phenotypic expression of osteoblast and the possible molecular mechanism in hypoxia environment.Methods MTT, Annexin V/PI double staining, alkaline phosphatase(ALP) activity assay and enzyme-linked immnosorbent assay(ELISA) were performed respectively to detect the effect of salidroside on regulating phenotypic expression of MG-63 cells exposed to hypoxia.Then RT-PCR and Western blot were conducted to detect the expression levels of Osterix and Runx2 which were related to differentiation.At last, we investigated the expression levels of components of the HIF-1α pathway by RT-PCR,Western blot and ELISA, respectively.Immunofluorescence confocal microscope technology and luciferase reporter assay were performed to explore nuclear translocation and transcriptional activity of HIF-1α.Results Salidroside could markedly promote MG-63 cell proliferation, inhibit hypoxia-induced apoptosis, stimulate cell differentiation and promote expression levels of components of the HIF-1α pathway in hypoxia environment.Conclusion Salidroside could regulate phenotypic expression of MG-63 cells through HIF-1α/VEGF signaling pathway in hypoxia environment.
