Effects of glucocorticoids on intracellular calcium in microglial cells
10.3969/j.issn.1001-1978.2017.06.026
- VernacularTitle:糖皮质激素对小胶质细胞内钙的影响
- Author:
Shuqiao HE
;
Xu QIAN
;
Guiping ZHANG
;
Weiwen ZHANG
;
Mei ZHANG
- Keywords:
glucocorticoid;
microglia;
α7nAChR;
calcium;
nicotine;
MLA
- From:
Chinese Pharmacological Bulletin
2017;33(6):878-883
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of hydrocortisone on intracellular calcium in microglial cells.Methods The intracellular calcium was measured by instantaneous scanning with confocal laser microscope(CLM) in BV-2 cells, and fluo3-AM was used to dye the intracellular calcium.Results Both hydrocortisone and nicotine could obviously increase intracellular calcium in BV-2 cells(P<0.05).It was indicated by instantaneous scanning with CLM that hydrocortisone induced the rising of intracellular calcium immediately, and reached the peak about at the fifteenth second, and sustained for 10 seconds, then declined to baseline at 200th second.The effect of hydrocortisone on intracellular calcium exhibited a highly consistency with nicotine.Antagonist of glucocorticoid receptors RU486 could not abolish the rising of intracellular calcium induced by hydrocortisone(P>0.05);but the blocker of α7 nicotinic acetylcholine receptor(α7nAChR) methyllycaconitine could suppress the rising of intracellular calcium induced by hydrocortisone(P<0.05).Conclusion Hydrocortisone enhances intracellular calcium via α7nAChR in microglial cells, which not only demonstrates the non-genomic effect of glucocorticoid, but also suggests that glucocorticoid could serve as endogenous ligand of α7nAChR.
