Effects of CXCR4 gene transfection on biologicalbehavior of bone marrow mesenchymal stem cells (in vitro)
10.3969/j.issn.1001-1978.2017.06.013
- VernacularTitle:CXCR4基因转染对骨髓间充质干细胞体外生物学行为的影响
- Author:
Yuying WANG
;
Nan ZHANG
;
Xiuli LI
;
Yameng WANG
;
Shaoheng LI
;
Yuhui YAN
;
Jie SONG
;
Jingxian YANG
;
Qingping WEN
- Keywords:
bone marrow mesenchymal stem cells;
chemokine receptor CXCR4;
gene transfection;
proliferation;
differentiation;
Migration
- From:
Chinese Pharmacological Bulletin
2017;33(6):806-813
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observate the effect of chemokine receptor(CXCR4) gene transfection on biological behavior of bone marrow mesenchymal stem cells in vitro.Methods Firstly, bone marrow mesenchymal stem cells were divided into three groups:GFP(transfected GFP into MSCs), CXCR4+(transfected CXCR4+ into MSCs) and CXCR4-(transfected CXCR4-into MSCs) group.Then, their capacity of proliferation, differentiation and migration ability (in vitro) was assessed with immunofluorescence cytochemistry method, flow cytometry assay and Transwell cell chemotaxis test.Results The high or low expression of CXCR4 had no effect on their ability of proliferation and differentiation into lung tissue.Compared with GFP group, however, CXCR4+-MSCs group significantly increased the number of migrating cells, while CXCR4——MSCs group showed no change in the number of migrating cells.Conclusions The proliferation and differentiation capacities are not affected by the high or low expression of CXCR4.The high expression of CXCR4 can significantly enhance the migration ability of MSCs to inflammatory lesions, and the low one has no effect on the migration of the cells.After the transplantation of MSCs, CXCR4′s high expression will access to the lesion area to participate in tissue repairing rapidly and largely, significantly enhancing the therapeutic efficacy.
