Effect of myricetin on migation and invasion ofhepatoma SMMC-7721 cells
10.3969/j.issn.1001-1978.2017.06.016
- VernacularTitle:杨梅素对肝癌SMMC-7721细胞迁移及侵袭影响的研究
- Author:
Lei ZHU
;
Weigan SHENG
;
Zhengxin XU
- Keywords:
EMT;
myricetin;
SMMC-7721 cell;
cell migration;
cell invasion;
cytoskeleton
- From:
Chinese Pharmacological Bulletin
2017;33(6):823-829
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of myricetin on the migration and invasion of hepatoma SMMC-7721 cells.Methods SMMC-7721 cells were treated with different concentrations of myricetin.Migration and invasion of SMMC-7721 cells were examined by Wound healing and Transwell, and the expression of E-cadherin and N-cadherin was detected by RT-qPCR and Western blot.Results Myricetin may inhibit the viability of SMMC-7721 cells via different concentration(10~40 μmol·L-1);furthermore, myricetin might also inhibit the migration and invasion of hepatoma SMMC-7721 cells.Meanwhile with the increasing of myricetin concentration, both filopodia and lamellipodia formation was reducd, and 7721 cells displayed in more integrity.And data from RT-qPCR and Western blot demonstrated that myricetin may up-regulate the expression of E-cadherin, simultaneously, down-regulate N-cadherin in SMMC-7721 cells.Conclusion Myricetin may influence the cell migration and invasion through up-regulating the expression of E-cadherin, simultaneously, down-regulating N-cadherin and activate cytoskeleton remolding of SMMC-7721.
