Effect of Rhizoma drynariae drug-containing serumon osteogenic differentiation of bone marrowmesenchymal stem cells by wnt/beta-catenin signaling pathway
10.3969/j.issn.1001-1978.2017.06.017
- VernacularTitle:骨碎补含药血清经wnt/β-catenin信号通路对大鼠骨髓间充质干细胞成骨分化的影响
- Author:
Yungang CHEN
;
Guoqing TAN
;
Weilong REN
;
Zhanwang XU
- Keywords:
Rhizoma drynariae;
containing serum;
bone marrow mesenchymal stem cells;
wnt/β-catenin;
proliferation;
alkaline phosphatase;
osteogenesis differentiation
- From:
Chinese Pharmacological Bulletin
2017;33(6):830-836
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the drug-containing serum of Rhizoma drynariae on osteogenesis differentiation of bone marrow mesenchymal stem, and discuss the possible mechanism.Methods BMSCs were cultured in media with different concentrations of medicine containing serum.BMSCs proliferation ability was detected in 3,5,7,9 days by CCK-8.ALP activity was detected after 7,10,14 days′ induction.After 3 weeks culturing, alizarin red staining was performed to observe the formation of calcium nodules.The expression of β-catenin,LRP5,RUNX-2 and Osteriex mRNA were detected using RT-PCR.The protein expression of β-catenin,LRP5 was detected using Elisa method.Results Rhizoma drynariae drug-containing serum could obviously promote the proliferation of BMSCs and calci-fied nodule formation.Besides, the ALP activity was improved in a certain period of time.The expression of β-catenin,LRP5,GSK-3β,RUNX-2 and Osteriex mRNA were significantly up-regulated,and the protein expression of β-catenin,LRP5 was up-regulated too.The expression of GSK-3β was down-trgulated.Conclusions Rhizoma drynariae drug-containing serum promotes mineralization and osteogenic differentiationof BMSCs, and the mechanism is closely related with activating WNT/beta-catenin signaling pathway, raising the beta-catenin, LRP5, RUNX-2, and Osteriex mRNA expression, beta-catenin, LRP5 protein expression,and down-regulation of GSK-3β mRNA expression.
