Metformin attenuates bone cancer pain by inhibiting expression of p-STAT3
10.3969/j.issn.1001-1978.2017.06.019
- VernacularTitle:二甲双胍通过减少STAT3磷酸化缓解大鼠骨癌痛
- Author:
Anqi GE
;
Hong YIN
;
Dengfeng LI
;
Hui ZHANG
;
Wei ZHAO
;
Ming YAN
;
Bei MIAO
- Keywords:
bone neoplasms;
pain;
metformin;
signal transducers and activators of transcription 3;
phosphorylation;
spinal dorsal horn;
rat
- From:
Chinese Pharmacological Bulletin
2017;33(6):844-848
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of metformin on phospho-signal transducers and activators of transcription 3(p-STAT3) expression in the spinal dorsal horn in rats with bone cancer pain(BCP).Methods Healthy female SD rats weighing 200~220 g were randomly divided into four groups(n=12 each): Sham+NS, Sham+Metformin, BCP+NS, BCP+Metformin groups.Normal saline or metformin(200 mg·kg-1) was given intraperitoneally once a day at 7~14 day after surgery in four groups.Mechanical withdrawal threshold(MWT) was measured on-1, 3, 5, 7, 10, 12, 14 day after BCP.The L4-6 segments of spinal cords were used to detect the expression of p-STAT3 by Western blot and immunohistochemical staining technique after the behavioral test was completed on day 14.Results Intraperitoneal injection of metformin reversed hyperalgesia and suppressed the expression of p-STAT3 in BCP rats.Conclusion Metformin can attenuate BCP by inhibiting the expression of p-STAT3 in the spinal dorsal horn.
