Study on transcriptome of Oncomelania hupensis before and after Schistoso-ma japonicum invasion Ⅰ De novo assembly of data by RNA-Seq
10.16250/j.32.1374.2016204
- VernacularTitle:日本血吸虫侵袭前后的湖北钉螺转录组研究ⅠRNA-Seq数据的de novo拼接
- Author:
Zhiqiang QIN
;
Xiaojing MO
;
Wei GUAN
;
Shizhu LI
- Keywords:
Schistosoma japonicum;
Oncomelania hupensis;
RNA-seq;
de novo assembly;
Transcriptome
- From:
Chinese Journal of Schistosomiasis Control
2017;29(3):300-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To produce a comprehensive transcript dataset of Oncomelania hupensis before and after Schistosoma japonicum infection,so as to provide experimental data for perfecting genetic structural information and excavating related mo-lecular markers of O. hupensis infected by S. japonicum. Methods O. hupensis snails were divided into the following 3 groups:one week after S. japonicum miracidium infection,4 weeks after S. japonicum miracidium infection,and normal condition. Mil-lion high-quality reads were obtained from the normalized cDNA of the pooled samples,which were assembled into transcripts. Results A total of 63686 unigenes were identified and were classified into 4 main categories,including general functional pre-diction(15.36%),signal transduction mechanism(11.75%),posttranslational modification(8.89%),and functional unknown (12.20%). Conclusions The transcriptome information of O. hupensis snail after the invasion of S. japonicum shows that sever-al genes are significantly up-regulated or down regulated expression,and that the availability of transcriptome information might provide a strong foundation for further understanding the schistosome-snail interaction at the molecular level.
