Investigation on the Relationship of H2S and the Accumulation of Extracellular Matrix of the Diabetic Nephropathic Rats By BrdU labeling method
10.11969/j.issn.1673-548X.2017.05.028
- VernacularTitle:BrdU标记法探讨糖尿病大鼠H2S与细胞外基质积聚的关系
- Author:
Shen CHEN
;
Wen HUANG
;
Mei ZHANG
;
Guojuan ZHANG
- Keywords:
Hydrogen sulfide;
Diabetic nephropathy;
α-Smooth muscle actin (α-SMA)
- From:
Journal of Medical Research
2017;46(5):112-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of hydrogen sulfide (H2S) and the extracellular matrix of the diabetic nephropathic rats' glomerulars.Methods Twenty-one male rats(Sprague-Dawley) were randomly divided into Control group(n =7),DN group (n =7) and DN + H2 S group (n =7).Diabetes was induced in the rats except Control group rats by STZ (60mg/kg).The rats of the DN + H2S group were injected with NaHS every day,and rats of other groups were injected with NS.Eight weeks later,three groups were compared in proteinuria of 24 hours,KW/BW,the proportion of the BrdU positive cell and the distribution of α-SMA in glomerulars.Results Compared with Control group,DN group rats were significantly increased in proteinuria of 24 hours and KW/BW (P ＜ 0.01).Kidney biopsy in DN groups rats were typical DN pathological manifestation.In treatment with NaHS,the DN rats in the DN + H2S group were decreased in proteinuria of 24 hours,KW/BW,the proportion of the BrdU positive cell and the distribution of α-SMA in glomerulars (P ＜ 0.01),and was similar with the control group in the pathology of renal.Conclusion The H2 S control the multiplication of myofibroblast,and were decreased in the distribution of α-SMA in glomerulars.The H2S treatment may put off the progress of the extracellular matrix in the DN rats' glomerulars.
