Effect of miR-340-5p targeting at BMP4 on differentiation of neural stem cells in rats
10.3760/cma.j.issn.1674-6554.2017.05.003
- VernacularTitle:靶向骨形态发生蛋白4的miR-340-5p对大鼠神经干细胞分化的影响
- Author:
Xueping HUANG
;
Wenyou WU
;
Handong ZHENG
;
Weixia YE
;
Dan LUO
- Keywords:
miR-340-5p;
Bone morphogenetic protein 4;
Neural stem cells;
Differentiation
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(5):395-399
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore regulation effect of miR-340-5p on regulating bone morphogenetic protein 4(BMP4) expression and the differentiation of rat's NSCs.MethodsNSCs of rats were divided randomly into three groups: normal group (Mock),group with nonsense oligonucleotide (Anti-Con) and group with antisense oligonucleotide of miR-340-5p (Anti-miR-340-5p).The qRT-PCR was used to detect the relative expression of miR-340-5p.The expression of NF200 and MAP-2 was detected by immunocytochemical staining and immunofluorescence,respectively.Western blot was used to detect the expression of BMP4 protein.ResultsThe relative levels of miR-340-5p expression were significantly decreased in Anti-miR-340-5p group (0.14±0.01) compared with that of Mock group(1.01±0.17) and Anti-Con group(1.07±0.13) (P<0.01).Immunocytochemical staining indicated that NF200 was positive in cells of Anti-miR-340-5p group.The proportion of MAP2 positive cells was increased in Anti-miR-340-5p group compared with other groups (P<0.05).Western blot showed the increased expression of BMP4 protein in Anti-miR-340-5p group (0.84±0.09) compared with Mock group(0.53±0.04) and Anti-Con group (0.63±0.09) (P<0.05).ConclusionThe miR-340-5p may exert a potential function in regulating differentiation of NSCs into neurons through a negative regulation of BMP4.
