Influence on Proliferation and Invasion of Human Breast Carcinoma Cell Line MDA-MB-231 by Using MicroRNA Interference Technique to Suppress MTDH Expression

VernacularTitle:microRNA干扰抑制MTDH的表达对乳腺癌细胞MDA-MB-231增殖和侵袭能力的影响
Author: Jiezhi SU ; Qingmo LIANG
Keywords: MDA-MB-231; MTDH gene; Transfection; RNAi
From: Journal of Medical Research 2017;46(5):84-90
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of RNA interference technique silencing MTDH on proliferation,metastasis and invasion of human breast cancer cell MDA-MB-231.Methods The MTDH miRNA was transfected into Human breast cancer cell MDA-MB-231 by means of lipofectamine 2000.The silencing efficiency of MTDH was examined by RT-PCR and western blot.The influence of MDA-MB-231 cell proliferation,metastasis and invasion ability from inhibition MTDH gene was conducted by using MTT assay,scratch experiments,and Transwell assay.Results MTDH miRNA effectively inhibited MTDH mRNA and protein levels.The best inhibition rate were 79.41％ and 80.56％ (P ＜ 0.05).With miRNA interferring cells,the ability of cells proliferation was decreased (P ＜ 0.05),and cells invasive and metastasis were depressed (P ＜ 0.05).Conclusion MTDH expression is obviously suppressed after transfected by MTDH miRNA in MDA-MB-231 cells.The inhibition of MTDH expression from microRNA leads to significant suppression of proliferation,invasion and metastasis of breast cancer cell MDA-MB-231.