Effect of docosapentenoic acid on the neurite outgrowth in PC12 cells

VernacularTitle:二十二碳五烯酸对PC12细胞神经突起生长的诱导作用
Author: Guanghui WANG ; Ming ZHONG ; Minna ZHANG ; Tingting WANG ; Yuting LIANG ; Dongrui LIANG ; Yan REN ; Chen ZHONG ; Jiantu CHE ; Jinglong WU
Keywords: Docosapentenoic acid; PC12 cells; Neurite outgrowth; βⅢ-tubulin; Extracellular regulated protein kinase; Kinase B
From: Chinese Journal of Behavioral Medicine and Brain Science 2017;26(5):390-394
CountryChina
Language:Chinese
Abstract: ObjectiveTo explore the inductive action of docosapentenoic acid(DPA) on neurite outgrowth in PC12 cells in vitro.MethodsNeurite outgrowth in PC12 cells was examined after the treatment with different concentration of DPA using Motic Zamges Plus software mapping cell image system.Western blot was performed to detect the expression of β Ⅲ-tubulin regulated protein kinase,a neuronal marker as well as ERK and protein kinase B (Akt) phosphorylation.ResultsPC12 cell neurite formation rate was increased in a concentration dependent manner in the induction of DPA,increased by 2.4% (DPA 10 μg/ml,P>0.05),18.6% (DPA 30 μg/ml,P<0.05) and 25.0% (DPA 50 μg/ml,P<0.05) compared with that in the control group.DPA promoted the expression of β Ⅲ-tubulin (P<0.05) and the phosphorylation level of ERK and Akt (P<0.05,P<0.01).ConclusionDPA promotes PC12 cell neurites growth and its mechanism may be related to the activation of ERK and Akt signaling pathways.