Expression of c-fos and c-jun in traumatic brain injury combined with tibial fracture in rats
10.3760/cma.j.issn.1671-7600.2017.05.011
- VernacularTitle:原癌基因c-fos和c-jun在创伤性脑损伤合并胫骨骨折大鼠中的表达
- Author:
Shengli ZHANG
;
Lingxia XIE
;
Lijun LIU
- Keywords:
Craniocerebral trauma;
Tibial fractures;
Gene expression;
Fracture healing;
Wounds and injuries
- From:
Chinese Journal of Orthopaedic Trauma
2017;19(5):429-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize the expressiou of c-fos and c-jun in traumatic brain injury combined with tibial fracture in rats.Methods One hundred and twenty male SD rats were randomized into equal 2 groups (n =60).Models of traumatic brain injury combined with tibial fracture were established in the experimental group while models of simple tibial fracture in the control group.After animal cerebral cortex and tissues of fracture proximal ends were sampled,qPCR techniques were used to detect the mRNA expression levels of c-fos and c-jun at 6 time points of 1 h,3 h,5 h,1 w,3 wand4w (n=10) in both experimental and control groups.Results There were no significant differences between the 2 groups in the expression levels of c-jun or c-fos gene in the cerebral cortex or tibial samples at 1 h,3 h,5 h,1 w,3 w or 4 weeks after fracture (P ＞ 0.05).The expression of c-fos in the cerebral cortex peaked at 1 h in both groups (8.98 ± 3.06 & 8.48 ± 1.74),showing significant differences between the peak values and the low levels at other time points (P ＜ 0.05).The expression of c-jun in the cerebral cortex peaked at 4 w in both groups (3.94 ±0.59 & 3.91 ±0.41),showiug significant differences from other time points (P ＜ 0.05).The expression of c-fos in the tibial samples peaked at 1 w in both groups (1.99 ±0.27 & 1.94 ±0.27) and that of c-jun did too at 1 w in both groups (3.95 ±0.41 & 3.86 ±0.43),showing significant differences from other time points (P ＜ 0.05).Conclusion The high expression levels of c-fos and c-jun in the brain tissue may not be associated with the high expression levels of c-fos and c-jun in the fracture region.
