A natural pancreatic 3D bioscaffold platform for in vitro study on exocrine pancreas
10.3760/cma.j.issn.1007-8118.2017.05.013
- VernacularTitle:基于天然胰腺脱细胞三维支架的外分泌胰腺体外实验
- Author:
Xin WANG
;
Zhao LI
;
Yueguang LI
;
Kun ZHANG
;
Yue DU
- Keywords:
Acellular scaffold;
Acellular matrix;
Pancreas;
Acinar cell;
Proliferation;
Differentiation
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(5):332-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the biochemical function of rat nature three-dimensional acellular pancreatic bioscaffold (3D-APB) in promoting cell proliferation and differentiation in vitro.Methods The fresh pancreas from 10 rats were perfused to prepare 3D-APB.The biocompatibility of 3D-APB was eva luated.The experiment was divided into 4 groups based on 4 kinds of three-dimensional media for AR42J culture,including blank control group,ECM group,PLGA group and 3D-APB group.We compared the proliferation and differentiation of AR42J pancreatic acinar cell at 3 days,5 days,7 days and 10 days after seeding among 4 groups.Results The 3D-APB could represent a biocompatible scaffold capable of integrating within host tissue.The proliferation rate of AR42J cells by MTT in 3D-APB was higher,while the apoptosis rate by flow cytometry was lower than those in other 3 media,which were all significantly different (P ＜ 0.05),respectively.The protein expression of PDX-1 and PTF-1 by western blot in 3D-APB group was greatly higher than those in other 3 groups (both P ＜ 0.05).The mRNA expression of PDX-1 and PTF-1 through qRT-PCR was significantly higher than those in other 3 groups (both P ＜ 0.05).Conclusion Compared with the commonly used chemical and natural scaffold at present,3D-APB could promote cell proliferation and differentiation,which was more appropriate for regenerative medicine.
