Role of nerve growth factor in the differentiation and proliferation of regulatory T cells induced by mesenchymal stem cells

VernacularTitle:神经生长因子增强间充质干细胞诱导CD4+CD25+Treg细胞分化和增殖的作用
Author: Wang LONG ; Bingyi SHI ; Li XIAO ; Xubiao XIE ; Fenghua PENG ; Gongbin LAN ; Shaojie YU ; Yu WANG ; Helong DAI ; Chen GAO ; Longkai PENG
Keywords: Nerve growth factor; Mesenchymal stem cell; Regulatory T cell; Immune tolerance
From: Chinese Journal of Organ Transplantation 2017;38(2):112-115
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of nerve growth factor (NGF) on the ability of differentiation and proliferation of regulatory T cells (Tregs) induced by mesenchymal stem cells (MSCs).Methods The MSCs were stimulated directly by NGF.IL-10,TGF-β and HLA-G were tested.The expression of CD4 and CD25 was detected by flow cytometry after co-culture.The expression of CD4,CD25 and Foxp3 was detected by flow cytometry after Transwell co-culture.Results As compared with control group,the expression of IL-10,TGF-β and HLA-G in NGF group was increased (P＜0.05 for all).The number of Tregs was increased after the co-culture (P＜0.05).The reduction in IL-10 and TGF-β could block the inducing function of NGF (P＜0.05).Conclusion NGF can enhance the ability of differentiation and proliferation of Tregs induced by MSC,which is possibly associated with the increases in the expression of IL-10 and TGF-β.