The effect of basic fibroblast growth factor on the proliferation of gingiva-derived mesenchymal stem cells

VernacularTitle:碱性成纤维生长因子促进牙龈间充质干细胞增殖作用的研究
Author: Ying FANG ; Sujuan ZENG ; Xu WANG ; Lihong GE
Keywords: Basic fibroblast growth factor; Gingival-derived mesenchymal stem cells; Mesenchymal stem cells; Cell proliferation
From: Journal of Practical Stomatology 2016;32(5):603-606
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of basic fibroblast growth factor(bFGF) on the proliferation of gingiva-derived mesenchymal stem cells(GMSCs) in vitro.Methods:GMSCs were isolated from healthy gingival tissue samples and identified.GMSCs of passage 4 were treated by bFGF at 0,0.5,1,5,10,20 ng/ml respectively for 1-9 d.The proliferation of the cells was evaluated using CCK-8 kit.Results:bFGF at 0.5-20 ng/ml increased GMSCs proliferation.0.5-10 ng/ml of bFGF showed dose and time dependant proliferation promoting effect on GMSCs.Conclusion:bFGF can increase GMSCs proliferation ability in a dose and time dependant manner.