The effect of paclitaxel liposome on the apoposis of cancer cells in the lymph node with the metastasis of rabbit tongue cancer
10.3969/j.issn.1001-3733.2016.05.002
- VernacularTitle:紫杉醇脂质体对兔舌癌淋巴结转移癌细胞凋亡的研究
- Author:
Ligang ZHANG
;
Guilin HUANG
;
Xiaohua HU
;
Nini ZHANG
;
Jie YI
;
Li YAO
;
Zenian LIU
- Keywords:
Paclitaxel Liposome;
Free Paclitaxel Injection;
Cell apoptosis;
Target artery perfusion;
Regional lymph nodes
- From:
Journal of Practical Stomatology
2016;32(5):607-610
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of paclitaxel liposome on the apoptosis of cancer cells in neck metastatic lymph node of rabbit tongue cancer.Methods:24 New Zealand rabbits with VX-2 tumor at tongue edge were divided into 6 groups randomly after neck lymph node metastasis.Paclitaxel liposome,free paclitaxel and 5％ glucose were injected into the lingual arterial and ear marginal vein,respectively.A week after chemotherapy metastasis lymph nodes were collected.The apoptosis of the cancer cells in the lymph nodes was examined by transmission electron microscope,flow cytometry,and SP immunohistochemical method for P53 protein expression of the cancer tissue.Results:The apoptosis rate of cancer cells in the intra-arterial chemotherapy group was higher than that in the intra-venous chemotherapy group,in the paclitaxel liposome injection group was higher than that in the free paclitaxel injection group(P ＜ 0.05).The positive rates of P53 protein expression in paclitaxel liposome group by intra-arterial injection was the lowest (P ＜ 0.05).Conclusion:Paclitaxel liposome is more effective than free paclitaxel,regional arterial infusion is more effective than intra-venous infusion in the apoptosis induction of lymph node metastasis cancer cells.
