Deoxyadenosine antagonizes MTX-induced suppression of inflammatory bone destruction
10.3969/j.issn.1001-3733.2016.05.003
- VernacularTitle:脱氧腺苷拮抗甲氨蝶呤对炎性骨破坏的抑制作用
- Author:
Pengfei QU
;
Biao CHEN
;
Lin LEI
;
Yuhong HAO
;
Congmin LI
;
Lina GUO
;
Zhiyu JIA
;
Huaying LI
;
Lei YUE
;
Wei YANG
- Keywords:
Osteoclasts;
Methotrexate (MTX);
Deoxyadenosine (dAdo);
Bone destruction
- From:
Journal of Practical Stomatology
2016;32(5):611-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of deoxyadenosine(dAdo) on methotrexate (MTX) induced suppression of inflammatory bone destruction.Methods:The culture system of whole bone marrow cells (WBMCs) was utilized to evaluate osteoclastogenesis.TRAP staining and μCT analysis were utilized to evaluate osteoclastogenesis and bone destruction in adjuvant arthritis rats.Results:In the bone marrow culture system,MTX-induced suppression of osteoclastogenesis was abrogated by the addition of dAdo.dAdo canceled MTX-induced suppression of osteoclastogenesis in the rats with arthritis,and significantly abolished the therapeutic effects of MTX on inflammatory bone destruction in the rats.Conclusion:The accumulation of dAdo may be one cause of the losing effectiveness of MTX in bone destruction.
