The effect of barium titanate coating on the proliferation of mouse fibroblast L929 cells tested by MTT method
10.3969/j.issn.1001-3733.2016.05.004
- VernacularTitle:MTT法检测钛酸钡涂层对小鼠成纤维细胞L929增殖率的影响
- Author:
Xiaojin GUO
;
Wenhui WU
;
Jingya LIU
;
Dayan HU
;
Nailing CHEN
- Keywords:
Barium titanate coating;
MTT method;
Mouse fibroblast L929 cells
- From:
Journal of Practical Stomatology
2016;32(5):615-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of the leaching liquids of the pure titanium porcelain crowns with barium titanate coating on the proliferation of L929 cells,and to evaluate its cytotoxicity level.Methods:The L929 ceils were cultured in vitro with leaching liquids of titanium porcelain specimens with barium titanate coating (group A),and titanium porcelain specimens(group B) for 1,3 and 5 days respectively.RPMI1640 containing 10％ fetal bovine serum and 1％ phenol was served as the negative(group C) and positive control group(group D),respectively.MTT method was used to test the effects of barium titanate coating on the proliferation of mouse fibroblast L929 cells,the cytotoxicity of the 4 groups was graded.Results:L929 cells of the group showed normal morphology and vigorous growth except group D.During the whole experiment,the absorbance values of group A was greater than that of group C(P ＜0.05).The cytotoxic gradation of group A grade 0.Conclusion:Titanium porcelain specimens with barium titanate coating has a good cytocompatibility.
