The role of cAMP signaling on the differentiation of stem cells from the apical papilla

VernacularTitle:cAMP信号通路在根尖牙乳头干细胞定向分化中的作用
Author: Shengzhe SU ; Yongna ZHU ; Jing ZHANG ; Song LI
Keywords: Stem cells from the apical papilla; cAMP; Differentiation
From: Journal of Practical Stomatology 2016;32(5):650-654
CountryChina
Language:Chinese
Abstract: Objective:The aim of this study was to investigate the role of cyclic adenosine monophosphate (cAMP) signaling on the differentiation of stem cells from the apical papilla (SCAPs).Methods:SCAPs were cultured by enzyme digestion method.After adding activator (Forskolin) and inhibitor (H-89) into the culture of SCAPs in mineralization-inducing medium,calcium deposition was measured by alizarin red staining.QPCR was performed to measure the mRNA expression of mineralization-related genes.Results:When the cAMP signaling was activated,calcium deposition and the mRNA expression of ALP,OCN,OSX and RUNX2 increased sharply.After inhibiting the signaling,calcium deposition and the mRNA expression of ALP,OCN,OSX and RUNX2 decreased.Conclusion:The cAMP signaling may promote the odontogenic and osteogenic differentiation of SCAPs.