Protective role of GLP-1 on AOPPs-induced apoptosis of cardiomyocytes
10.3969/j.issn.1006-6187.2017.06.016
- VernacularTitle:胰升血糖素样肽1对晚期氧化蛋白产物诱导的心肌细胞凋亡保护作用的研究
- Author:
Zhouyi XIONG
;
Bin XIONG
;
Jianneng WU
- Keywords:
Advanced oxidation protein products;
Glucagon-like peptide-1;
Apoptosis;
PI3K/Akt/Bad pathway
- From:
Chinese Journal of Diabetes
2017;25(6):558-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective role and mechanisms of glucagon-like peptide-1(GLP-1) on advanced oxidation protein products (AOPPs)-induced apoptosis of cardiomyocytes. Methods The H9C2 cells were selected in this study and divided into blank control group,RSA control group,and groups treated with indicated concentrations of AOPPs with or without GLP-1,and AOPPs +GLP-1+LY294002 for 24 hours respectively. Cell viability was detected by CCK-8 assay. The ROS level was detected by DCFH-DA fluorescent probe. The cell apoptosis was tested by fluorescent staining and flow cytometry. The expression of p-Akt,p-Bad,Bcl-2,Bax,and active-caspase-3 proteins were evaluated by Western blot. Results GLP-1 attenuated AOPPs-induced cytotoxicity[(0.929±0.083) vs (1.409±0.099),P<0.01],decreased AOPPs-induced ROS[(47.817±0.878)% vs (25.413±2.597)%,P<0.01] and apoptosis[(15.773±3.130)% vs (9.715±0.757)%,P<0.01]. GLP-1 improved AOPPs-induced phosphorylation of Akt and Bad,increased the expression of Bcl-2,and decreased the expression of Bax and the activation of caspase-3. Conclusion GLP-1 protects cardiomyocytes against AOPP-induced apoptosis,predominantly via the PI3K/Akt/Bad pathway.
