Relationship between Serum Diamine Oxidase Content and Intestinal Motility after Acute Spinal Cord Injury
10.3969/j.issn.1006-9771.2017.06.003
- VernacularTitle:血清二胺氧化酶含量与大鼠急性脊髓损伤后肠道运动功能的关系
- Author:
Sen YANG
;
Zheng YANG
;
Jingxin YAN
;
Zhou XU
;
Wenjian XU
;
Haiyan TIAN
;
Xiaolin DAI
;
Ping YANG
- Keywords:
spinal cord injury;
diamine oxidase;
intestinal motor function;
mucosal injury;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2017;23(6):634-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the level of diamine oxidase (DAO), and observe the intestinal motor and mucosal barrier injury after acute spinal cord injury (SCI) in rats. Methods A total of 45 Sprague-Dawley rats were randomly divided into SCI group (group A, n=15), sham group (group B, n=15) and control group (group C, n=15). SCI model was established with Allen's strike mode (10 g × 25 mm) by striking T10 spinal segment in rats. One day, three days and seven days after SCI, hind limb motor function was assessed with Basso-Beat-tie-Bresnahan (BBB) Scale in each group, the myoelectric slow wave and ileum smooth muscle contractility were measured in rats, ileum tis-sues were tested with HE staining, and the DAO content of serum was tested with ELISA kit. Results One day, three days and seven days af-ter SCI, the BBB score was significantly lower in group A than in groups B and C (P<0.001). One day, three days after SCI, the frequency and amplitude of both slow wave and contractility were lower in group A than in groups B and C (P<0.05);seven days after SCI, there was no significant difference among three groups (P>0.05). Group A showed ileal mucosal edema, lodging, inflammatory cell infiltration, and submucosal gap increase. The Chiu's score of intestinal mucosal injury was higher in group A than in groups B and C (P<0.05), as well as the serum DAO content one day and three days after SCI (P<0.05), and no significant difference was found in the serum DAO content among three groups seven days after SCI (P>0.05). Conclusion Serum DAO content may respond to the intestinal motor function and mu-cosal injury after acute SCI in rats.
