Simultaneous determination of seven constituents in Dangfei Liganning Capsules by HPLC
10.3969/j.issn.1001-1528.2017.06.016
- VernacularTitle:HPLC法同时测定当飞利肝宁胶囊中7种成分
- Author:
Yutang LONG
;
Hao CHEN
;
Jing LIU
- Keywords:
Dangfei Liganning Capsules;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(6):1187-1190
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of swertiamarin,gentiopicrin,swertianolin,taxifolin,silychristin,silydianin and silybin in Dangfei Liganning Capsules (Silybi Fructus and Swertiae Herba).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Kromasil C18 column (4.6 mm × 250 mm,5.0 μm),with the mobile phase comprising of methanol-0.1％ formic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 254 nm and 288 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.78％-100.45％ with the RSDs of less than 2.0％.CONCLUSION This accurate and reproducible method can be used for the rapid quality control of Dangfei Liganning Capsules.
