HPLC-ELSD fingerprints of oligosaccharide sites from mycelia of Hericium erinaceum solid cultures and Weilening Tablets
10.3969/j.issn.1001-1528.2017.06.022
- VernacularTitle:猴头菌丝固体培养物及胃乐宁片低聚糖部位的HPLC-ELSD指纹图谱
- Author:
Ting CHEN
;
Junhui YANG
;
Junsong LI
;
Kun CHEN
;
Liuqing DI
;
Qing GUO
- Keywords:
mycelia of Hericium erinaceum;
solid cultures;
Weilening Tablets;
oligosaccharide;
mannitol;
lactose;
trehalose;
HPLC-ELSD fingerprints
- From:
Chinese Traditional Patent Medicine
2017;39(6):1211-1216
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC-ELSD fingerprints of oligosaccharide sites from mycelia of Hericium erinaceum solid cultures and Weilening Tablets.METHODS The analysis of aqueous extract from samples was performed on a 80 ℃ thermostatic Waters XBridge TM Amide column (4.6 mm × 150 mm,3.5 μm),with the mobile phase comprising of acetonitrile-0.2％ ammonium acetate flowing at 1 mL/min in a gradient elution manner.RESULTS There were eight and nine common peaks in two HPLC-ELSD fingerprints with the similarties of 0.994-0.966 and 0.990-0.997,respectively.Three of them were mannitol,lactose and trehalose,which showed good linear relationships within their own ranges (r ≥ 0.999 0),the average recoveries were 95.08％-104.82％ with the RSDs of 1.12％-2.90％.CONCLUSION This simple and accurate method can be used for the rapid quality control of mycelia of Hericium erinaceum solid cultures and Weilening Tablets.
