In vitro synergistic effect of tetrandrine on ketoconazole against Candida parapsilosis complex
10.3760/cma.j.issn.0412-4030.2017.06.007
- VernacularTitle:粉防己碱对酮康唑抗近平滑念珠菌复合体的体外增效活性研究
- Author:
Yajing ZHAO
;
Shuixiu LI
;
Ling JIANG
;
Yanjun SONG
;
Hui GUO
;
Weida LIU
;
Yongnian SHEN
;
Kunju ZHU
;
Hong ZHANG
- Keywords:
Candida;
Ketoconazole;
Tetrandrine;
Drug synergism;
Candida parapsilosis complex
- From:
Chinese Journal of Dermatology
2017;50(6):417-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the in vitro synergistic effect of tetrandrine on ketoconazole against Candida parapsilosis complex.Methods According to the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines,the microdilution checkerboard method was used to evaluate in vitro antifungal activities of ketoconazole alone and in combination with tetrandrine against 21 clinical isolates of Candida parapsilosis complex based on the fractional inhibitory concentration index (FICI).Antifungal effects of the above drugs at different time points were evaluated by the XTT assay,and then time-killing curves were drawn and assessed to investigate the in vitro dynamic antifungal activity.Results The minimum inhibitory concentrations (MICs) of tetrandrine and ketoconazole alone against 21 clinical isolates of Candida parapsilosis complex were 32-64 mg/L and 0.031 25-2 mg/L,respectively.When ketoconazole was combined with tetrandrine,MICs of tetrandrine and ketoconazole were reduced to 2-8 mg/L and 0.008-0.25 mg/L respectively,and the FICI ranged from 0.09 to 0.5.The time-killing curves revealed that the fungal growth was delayed obviously in the combination group compared with the ketoconazole alone group and tetrandrine alone group.Conclusion Tetrandrine has obvious synergistic effects on ketoconazole against Candida parapsilosis complex in vitro.
