Studies on the serum peptide fingerprint of bacterial bloodstream infection by matrix-assisted laser desorption ionization time of flight mass spectrometry
10.11855/j.issn.0577-7402.2017.05.08
- VernacularTitle:应用基质辅助激光解吸电离飞行时间质谱研究细菌性血流感染的血清多肽指纹图谱
- Author:
Yating MA
;
Ming YANG
;
Shang HE
;
Chen CHEN
;
Kexin ZHANG
;
Chengbin WANG
- Keywords:
matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS);
bacterial bloodstream infection;
Escherichia coli;
Staphylococcus aureus;
serum peptide profiling
- From:
Medical Journal of Chinese People's Liberation Army
2017;42(5):402-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the serum peptide fingerprint using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technology,and find the different peaks with potential significance and establish the diagnosis model of Staphylococcus aureus and Escherichia coli bloodstream infection.Methods To establish ICR mice model of S.aureus and E.coli bloodstream infections,and collect serum samples.The serum samples were purified by weak cation exchange beads,the serum peptide fingerprint was recognized by using MALDI-TOF MS and BioExplorer software between infections group and normal control group.Results Compared with the normal control group,6 peptides were up-regulated,7 peptides downregulated and 8 peptides up-regulated first and then down-regulated in S.aureus infection group;And 5 peptides down-regulated,4 peptides down-regulated first and then up-regulated,and 8 peptides up-regulated first and then down-regulated in E.coli infection group.Conclusion MALDI-TOF MS combined with BioExplorer software may be used as a tool to study the serum peptides of S.aureus and E.coli bloodstream infection,effectively find significant peptides for establishing a diagnosis model of these two bacterial infections,and has a certain value for the diagnosis of bacterial bloodstream infection.
