Effect of trehalose on survival rate for fat cells after cryopreservation

VernacularTitle:海藻糖对脂肪细胞冻存后存活率的影响
Author: Ying DENG ; Shaoqian LIU ; Hongju XIE ; Fangfang TANG ; Ming LI ; Nian CHEN
Keywords: trehalose; autologous fat globules; freezing; preserve
From: Journal of Central South University(Medical Sciences) 2017;42(5):507-510
CountryChina
Language:Chinese
Abstract: Objective:To explore effects of trehalose as a cryopreserve agent on survival rate of fatty tissue after cryopreservation.Methods:The liposuction was used on the abdomen of adult female.After centrifugation and purification,adipose was randomized into the following three groups,the trehalose group,the fetal bovine serum (FBS)+ 10％DMSO group and the physiological saline group.The specimens were cryopreserved at-196 ℃ for 3 months and then the HE staining,glucose transfer method and CK method were used to detect the cell survival rate in each group.Results:The activity of adipose in the trehalose group and FBS+10％DMSO group adipose was higher than that in the physiological saline group (P＜0.05);while there was no significant difference between the trehalose group and FBS+10％DMSO group (P＞0.05).Conclusion:As cryoprotectant,trehalose could keep fat cell viability,and adipose tissue can be used for clinical transplantation after 3 months' freezing.