Effects of ING5 gene on the malignant phenotype of breast cancer Bcap-37 cells
10.11855/j.issn.0577-7402.2017.01.03
- VernacularTitle:ING5基因对乳腺癌细胞Bcap-37恶性表型的影响
- Author:
Yang SONG
;
Yizeng WAN
;
Shupeng ZHAO
;
Fengjie QI
;
Lei FANG
;
Jicheng WU
;
Shuai SHI
;
Huachuan ZHENG
- Keywords:
breast neoplasms;
inhibitor of growth;
Bcap-37 cells;
genes;
tumor suppressor
- From:
Medical Journal of Chinese People's Liberation Army
2017;42(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of inhibitor of growth 5 (ING5) gene on the proliferation, apoptosis, migration and cell cycle of human breast cancer Bcap-37 cells.Methods The eukaryotic ING5-expressing plasmid and GFP-empty plasmid were steadily transfected in Bcap-37 cells, the expression of green fluorescent protein was measured with fluorescence microscopy, and the high expression of ING5 was measured by real time-PCR. Bcap-37-ING5 cells served as the experimental group, Bcap-37-GFP cells as the mock group and Bcap-37 as the control group. The effects of ING5 on the proliferation were detected by MTT, the cell cycle and apoptosis were detected by Flow cytometry, and the cell migration was detected by cell wound scratch assay and Transwell experiment.Results Bcap-37 cell lines steadily expressing ING5 protein with GFP-tag were acquired by stable transfection. ING5 over-expression inhibited the proliferation and led to G2 arrest of Bcap-37 cells, increased cells apoptosis and decreased the cell migration ability (P<0.05).Conclusion ING5 over-expression may have reverse effect for malignant phenotype of breast cancer cells, and may be employed to indicate the biomarker of prognosis of breast cancer patients and regarded as a target of gene therapy.
