Expression of TET2 and DNMT3A in peripheral T cell lymphoma and their significance
10.13315/j.cnki.cjcep.2017.04.008
- VernacularTitle:外周细胞淋巴瘤中TET2和DNMT3A的表达及意义
- Author:
Mengqi SUN
;
Lili WANG
;
Wenwen RAN
;
Hong LI
;
Xiaoming XING
- Keywords:
lymphoma;
peripheral T cell lymphoma;
TET2;
DNMT3A;
immunohistochemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(4):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of TET2 and DNMT3A in patients with peripheral T cell lymphoma (PTCL) and the relationship to immunophenotypes of PTCL.Methods Using a panel of immunohistochemical markers (CD3,CD4,CD10,BCL-6,CXCL-13,CD30,ALK),all cases of PTCLs were further divided into four groups,including angioimmunoblastic T cell lymphoma (AITL),peripheral T cell lymphoma,not otherwise specified (PTCL-NOS),anaplastic lymphoma kinase negative anaplastic large cell lymphoma (ALK-ALCL) and anaplastic lymphoma kinase positive anaplastic large cell lymphoma (ALK + ALCL).The expression of TET2 and DNMT3A in 89 cases of PTCL was detected by immunohistochemical analysis.Results 89 cases were divide into four subtypes,AITL (36/89),PTCL-NOS (26/89),ALKALCL (18/89),and ALK + ALCL (9/89).Immunohistochemistry staining revealed higher cytoplasmic expression of TET2 and DNMT3A in AITL than that of in PTCL-NOS and ALCL (P ＜ 0.05).And the nuclear expression of DNMT3A in patients with AITL was higher than that of PTCL-NOS and ALCL (P ＜ 0.05).The cytoplasmic expression of TET2 was positively related with both cytoplasmic and nuclear expression of DNMT3A in patients with AITL (P ＜ 0.05).Conclusion TET2 combined with DNMT3A could be used as markers in AITL diagnosis,which could provide new strategy for AITL diagnosis.
