The therapeutic effect of low frequency ultrasound mediated microbubbles destruction on rabbit VX2 orthotopic hepatic tumors
10.3760/cma.j.issn.1004-4477.2017.06.015
- VernacularTitle:低频聚焦超声联合微泡治疗兔VX2原位肝癌移植瘤的疗效评价
- Author:
Yang SUN
;
Jieli LUO
;
Jianshe CHEN
;
Pintong HUANG
- Keywords:
Contrast-enhanced ultrasound;
Microbubbles;
Liver neoplasms,experimental;
Ultrasonic therapy
- From:
Chinese Journal of Ultrasonography
2017;26(6):535-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether low frequency ultrasound mediated microbubbles destruction (UMMD) could inhibit VX2 orthotopic hepatic tumor growth in rabbit models.Methods Twenty-four New Zealand white rabbits were implanted with VX2 tumor in left hepatic lobe to establish a homograft rabbit model of liver neoplasms in situ,which were randomly divided into four groups(6 rabbits in each group):group A (intravenous saline only),group B (intravenous microbubbles only),group C (intravenous saline+ low frequency focused ultrasound exposure),and group D (intravenous microbubbles+low frequency focused ultrasound exposure).After 3 days consecutive treatment,tumor volume(TV),and peak intensity (PI) were monitored by conventional ultrasound and contrast enhanced ultrasound(CEUS) on 0,1,7,14 and 21 days after treatment.The rabbits were euthanized at the end of the experiment.Tumor tissues were evaluated by HE stain.Results The parameters of TV and PI of each tumor had no significant difference among four groups before treatment(all P>0.05).TV had no significant difference among four groups on 1 day after treatment(all P>0.05);PI in group C and group D were significantly lower than those in group A and group B (all P<0.05).TV and PI in group D were significantly smaller than those in other groups on 7,14,21 days after treatment(all P<0.05).There was no statistical difference in TV and PI among group A,group B and group C(all P>0.05).The pathological changes of necrosis tissue,hemorrhagic damage of microvessel and thrombosis were observed in the tumors of group D only,whereas these changes occurred rarely in other groups.Conclusions UMMD can inhibit the growth of VX2 hepatic tumors in rabbits,and be used as a promising novel therapeutic strategy to liver neoplasms.
