Effects of Hesperidin on RAW264.7 Foam Cell Formation And Expression of ICAM-1

VernacularTitle:橙皮苷对RAW264.7巨噬细胞泡沫化及ICAM-1表达的影响
Author: Mingming FU ; Jiao GUO
Keywords: Hesperidin; RAW264.7 cells; ICAM-1; foam cells
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(3):474-479
CountryChina
Language:Chinese
Abstract: This study was aimed to investigate the effects of hesperidin on the macrophage foam cell formation in RAW264.7 cells and the expression of ICAM-1.RAW264.7 cells were culture in vitro and induced by oxLDL (50 μg· mL-1).Furthermore,cells were separated into the control group,oxLDL model group and hesperidin treatment group.The effect of hesperidin on cell viability in RAW264.7 was assessed by MTF assay.Oil Red O staining was examined by foam cells formation.Effect of hesperidin on protein expression of ICAM-1 was analyzed by western blot.In addition,the effect of hesperidin on mRNA expression of ICAM-1 was assessed by RT-PCR.The results showed that the viability should been over 80％ after less than or equal to 5 μM hesperidin treatment.Hesperidin decreased the protein expression of ICAM-1 in RAW264.7 cells.Additionally,hesperidin suppressed the mRNA expression of ICAM-1 in RAW264.7 cells.It was concluded that hesperidin can inhibit foam cell formation and the expression of ICAM-1 in RAW264.7 cells.It suggested that hesperidin protect against atherosclerosis.