The application of circulating tumor cells in early diagnosis of colorectal cancer metastasis
10.3760/cma.j.issn.1673-4904.2017.08.017
- VernacularTitle:外周血循环肿瘤细胞检测在结直肠癌转移早期诊断中的应用
- Author:
Ping YANG
;
Jianchang WEI
;
Tong ZHANG
;
Huacui CHEN
;
Shanqi ZENG
;
Jie CAO
- Keywords:
Colorectal cancer;
Neoplastic cells,circulating;
Metastasis;
Carcinoembryonic antigen
- From:
Chinese Journal of Postgraduates of Medicine
2017;40(8):735-737
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of circulating tumor cells (CTC) in peripheral blood of patients with different stages of colorectal cancer (CRC), and to evaluate its significance in early diagnosis of colorectal cancer metastasis. Methods Sixty patients with CRC (42 inⅡ-Ⅲstage and 18 in IV stage ) and 30 patients with benign rectal disease were recruited from January 2014 to December 2015. The CTC in peripheral blood was purified with Immunomagnetic Separation Technologie, and detected by immunofluorescence in situ hybridization (imFISH). The serum levels of CEA were detected by electrochemiluminescence method meanwhile. The correlation between CTC and CEA was analyzed. Results CTC positive rates in CRC patients were significantly higher than those in benign rectal disease controls. CTC positive rates inⅣstage were significantly higher than those inⅡ-Ⅲ stage. The expression of CTC was significantly correlated with CEA (r = 0.6652, P < 0.01). Conclusions The expression of CTC in CRC patients is significantly higher than that in benign rectal disease control group. It is closely related to clinical stages. Detection of peripheral blood CTC has important clinical significance in the early diagnosis of CRC metastasis.
