Preparation of Puerarin Cubic Phase and Study on Its in vitro Transdermal Characteristics under Mi-croneedle
10.6039/j.issn.1001-0408.2017.22.29
- VernacularTitle:葛根素立方液晶的制备及在微针作用下的体外透皮特性研究
- Author:
Jianmin MO
- Keywords:
Puerarin;
Cubic phase;
Preparation;
Microneedle;
in vitro transdermal
- From:
China Pharmacy
2017;28(22):3132-3135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare the puerarin cubic phase,and study its in vitro transdermal characteristics under micronee-dle. METHODS:Using glycerol monooleate,ethanol,puerarin and purified water as materials,injection method was used to pre-pare the puerarin cubic phase. Polarizing microscopy and small angle X-ray diffraction were used for characterizing. The in vitro transdermal characteristics of Puerarin 10% ethanol solution and puerarin cubic phase were comparatively studied by modified Franz diffusion cell,and the in vitro transdermal characteristics of puerarin cubic phase under microneedle were studied. RE-SULTS:Prepared puerarin cubic phase had no obvious phase distribution under polarizing microscope with dark green color,show-ing isotropic trait. The ratio of scattering peak position was √6:√8,indicating that its internal structure was spiral lattice. The trans-dermal rates of Puerarin 10%ethanol solution and puerarin cubic phase were 15.306,29.101μg/(cm2·h),and the cumulative trans-dermal amounts within 20 h were 190,545 μg/cm2,respectively. After the pretreatment on skin by 200 μm microneedle,the trans-dermal rates of puerarin cubic phase was 78.15 μg/(cm2·h),and the cumulative transdermal amounts within 20 h was 1450 μg/cm2. CONCLUSIONS:Puerarin cubic phase is successfully prepared,which shows stronger transdermal ability than puerarin ethanol aqueous solution and better transdermal ability when combined with microneedle administration.
