Laboratory diagnosis of the first imported case of Zika virus infection from Suriname into Guangdong,China
10.3969/j.issn.1002-2694.2017.06.010
- VernacularTitle:广东首例苏里南输入性寨卡病毒感染病例的实验室检测
- Author:
Jieyi LIANG
;
Jun DAI
;
Donghong LI
;
Yongxia SHI
;
Jicheng HUANG
;
Shuai YUAN
;
Kui ZHENG
;
Xiaobo LI
;
Xianguang ZHANG
;
Wei SONG
;
Huiming WU
- Keywords:
Zika virus;
real-time fluorescent RT-PCR;
nucleic acid detection;
phylogenetic analysis
- From:
Chinese Journal of Zoonoses
2017;33(6):522-525
- CountryChina
- Language:Chinese
-
Abstract:
We detected Zika virus (ZIKV) in a febrile case returning from Suriname and entry China from Guangzhou Baiyun International Airport Port.Serum and saliva samples were collected from a suspected case returning from Suriname.We detected ZIKV RNA using real-time fluorescence RT-PCR methods by both in-house reagent and commercial detection kits.RT-PCR detection was carried out with saliva sample and sequence analysis was performed.Phylogenetic tree was constructed to analyze the source of imported cases.Real-time fluorescent RT-PCR result showed that saliva was detected ZIKV RNA positive while for serum was weakly positive.A specific 1 500 bp fragment in size was amplified with saliva sample by RT-PCR.Sequence analysis showed 99％ homologous to the corresponding sequence of Brazil ZIKV (GenBank No.KX197250).Phylogenetic tree indicated it was located on African lineage.According to the epidemiological investigation results,clinical manifestations and nucleic acid detection of case,the suspected case was confirmed to infect Zika virus,being the first case from Suriname into Guangdong Province.
