Identification of a pathogenic Aeromonas veronii isolated from rock carp, Procypris rabaudi
10.3969/j.issn.1002-2694.2017.06.011
- VernacularTitle:岩原鲤致病性维氏气单胞菌的分离与鉴定
- Author:
Chengke ZHU
;
Guijia LIU
;
Zhengshi ZHANG
;
Decheng PU
;
Long ZHU
;
Chaowei ZHOU
;
Luo LEI
;
Zonglin ZHENG
- Keywords:
Aeromonas veronii;
Procypris rabaudi;
pathogenity;
antibiotic susceptibility test
- From:
Chinese Journal of Zoonoses
2017;33(6):526-534
- CountryChina
- Language:Chinese
-
Abstract:
In June 2016,a disease among the cultured rock carp (Procypris rabaudi) in Yongchuan of Chongqing Municipality occurred.The aim of this study was to investigate biological characteristics and provide reference for Aeromonas veronii identification diagnosis and treatment.Pathogenic bacteria strain YY01 from the dying fishes were examined and isolated.Strain YY01's taxonomic status was identified by observing the morphology,studying the physiological and biochemical characters and sequencing the 16S rRNA and housekeeping gene gyrB.Its pathogenicity was checked by artificial infection experiment and virulence genes.Furthermore,effective medicine was detected by drug sensitivity.The 16S rRNA and gyrB gene sequence of the strain YY01 was more than 99％ homology with that of Aeromonas veronii,suggesting that the pathogen was Aeromonas veronii,which was also identified by the results of biochemical analysis.The LD50 of strain YY01 to rcok carp was 5.06 × 104 CFU/g.Four virulence genes were detected from YY01,including aerolysin (aer),hemolysin (Hly),Outer Membrane Protein Gene A (OmpA) and adhesion (Aha) genes.Antibiotic sensitivity assays showed that among 40 antibiotics tested,22 were sensitive and 11 were resistant.In conclusion,the strain YY01 is identified as Aeromonas veronii and it is proved to have strong pathogenicity.
