Correlation between the expression of activator protein-1 (c-Fos/c-Jun) mRNA and gingival inflammation
10.3760/cma.j.issn.1673-4181.2017.03.011
- VernacularTitle:激活蛋白-1(c-Fos/c-Jun) mRNA水平与牙龈炎症相关性研究
- Author:
Dongwang ZHU
;
Ling GAO
;
Jiashan LI
;
Shaoyun JIANG
- Keywords:
Activator protein-1;
c-Fos;
c-Jun;
Gingival inflammation;
Gingival index
- From:
International Journal of Biomedical Engineering
2017;40(3):197-200,215
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation between the expression of activator protein-1 (c-Fos/c-Jun) mRNA and gingival inflammation,so as to discuss the pathogenesis of periodontitis.Methods The gingival tissues were divided into three groups according to the gingival index (GI),including GI=0 group (control group,14 cases),GI=1 group (15 cases) and GI=2 group (11 cases).The total RNA in each gingival tissue was extracted,and cDNA was synthesized by reverse transcription synthesis.The expressions of c-Fos and c-Jun mRNA in healthy gingival tissue (GI=0 group) were detected by reverse transcription-polymerase chain reaction.The levels of c-Fos and c-Jun mRNA in all the groups were detected by real-time quantitative PCR.Results Both c-Fos and c-Jun mRNA was expressed in healthy gingival tissues.The levels of c-Fos and c-Jun mRNA in GI=1 group was 15.58±9.19 and 3.47± 1.77,respectively,which was significantly higher than 1.31±1.03 and 1.32±0.94 in GI=0 group,and the differences were statistically significant (all P＜0.05).The level of c-Fos mRNA in GI=2 group was 3.01±1.48,which was lower than that in GI=1 group (P＜0.05) and higher than that in GI=0 group (P＜0.05).The level of c-Jun mRNA in GI=2 group was 1.48±0.65,which was lower than that in GI=1 group,and had no significant difference with GI=0 group (P＞ 0.05).Conclusions Activator protein-1 (c-Fos/c-Jun) is associated with the degree of gingival inflammation,suggesting that it is involved in the occurrence and development of gingival inflammation.
