Identification of Genomic Aberrations by Array Comparative Genomic Hybridization in Patients with Aortic Dissections.
10.5090/kjtcs.2011.44.2.123
- Author:
Jong Hui SUH
1
;
Jeong Seob YOON
;
Jong Bum KWON
;
Hwan Wook KIM
;
Young Pil WANG
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Incheon St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Korea. jsyoon@catholic.ac.kr
- Publication Type:Multicenter Study ; Original Article
- Keywords:
Aorta;
Aortic dissection;
Genes;
Polymerase chain reaction
- MeSH:
Aorta;
Cohort Studies;
Comparative Genomic Hybridization;
DNA;
Humans;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2011;44(2):123-130
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The aim of the present study was to identify chromosomal loci that contribute to the pathogenesis of aortic dissection (AD) in a Korean population using array comparative genomic hybridization (CGH) and to confirm the results using real-time polymerase chain reaction (PCR). MATERIALS AND METHODS: Eighteen patients with ADs were enrolled in this study. Genomic DNA was extracted from individual blood samples, and array CGH analyses were performed. Four corresponding genes with obvious genomic changes were analyzed using real-time PCR in order to assess the level of genomic imbalance identified by array CGH. RESULTS: Genomic gains were most frequently detected at 8q24.3 (56%), followed by regions 7q35, 11q12.2, and 15q25.2 (50%). Genomic losses were most frequently observed at 4q35.2 (56%). Real-time PCR confirmed the results of the array CGH studies of the COL6A2, DGCR14, PCSK6, and SDHA genes. CONCLUSION: This is the first study to identify candidate regions by array CGH in patients with ADs. The identification of genes that may predispose an individual to AD may lead to a better understanding of the mechanism of AD formation. Further multicenter studies comparing cohorts of patients of different ethnicities are warranted.
