A mouse cerebral cortical microinfarct model induced by ultrashort laser irradiation wih two-photon microscopy
10.3760/cma.j.issn.1673-4165.2017.05.008
- VernacularTitle:双光子显微镜飞秒激光照射建立小鼠大脑皮质微梗死模型
- Author:
Taotao SHI
;
Shijian LUO
;
Chaogang TANG
;
Xiaofeng CHEN
;
Yukun FENG
;
Ruxun HUANG
;
Zhong PEI
;
Zhendong LI
- Keywords:
Cerebral Infarction;
Cerebral Cortex;
Microscopy;
Confocal;
Neurons;
Cell Death;
Inflammation;
Tyrosine;
Disease Models;
Animal;
Mice
- From:
International Journal of Cerebrovascular Diseases
2017;25(5):425-430
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo verify the reliability of the mouse model of cerebral cortical microinfarct induced by two-photon microscopy and to explore its pathological changes.MethodsSeventeen male C57BL/6J mice were randomly divided into a microinfarct group (n=11) or a sham operation group (n=6).A thinned cranial window of 3 mm diameter was performed over the cerebral cortex with a high-speed micro-drill until the small blood vessels were clearly observed under a dissecting microscope.Then, a permanent single cortical penetrating arteriole occlusion was induced with a gradually enhanced ultrashort laser irradiation through the thinned cranial window with two-photon microscopy.At 7 days after modeling, the cerebral microinfarct volume was measured with HE staining, and the neuron loss, activation of glial cells and deposition of 3-nitrotyrosine were assessed using immunohistochemistry.ResultsThe target vessels of cerebral cortex in 8 (72.7%) mice were occluded and the microinfarcts formed in the microinfarct group, and the average microinfarct volume was 317.23±20.29 μm3.There were remarkable neuron loss and microglia infiltration in the infarcted core, a large number of reactive astrocytes surrounding the infarcted lesion, and massive deposition of 3-nitrotyrosine in the peri-infarct area.No infarcts were observed in the sham operation group.The deposition of 3-nitrotyrosine in the sham operation group was significantly less than that in the microinfarct group (8.00±1.48 vs.98.38±9.10;t=23.962, P<0.001).Conclusions The mouse model of cerebral cortical microinfarct induced by two-photon microscopy is reliable, and its histopathologic changes are consistent with the pathologic features of cerebral microinfarct.
