Simultaneous determination of thirteen saponins in Shusanqi Powder by HPLC
10.3969/j.issn.1001-1528.2017.06.014
- VernacularTitle:HPLC法同时测定熟三七散中13种皂苷
- Author:
Zexuan YU
;
Xiangxiang DAI
;
Shuangyou DU
;
Rengang MAO
;
Xianrong WU
- Keywords:
Shusanqi Powder;
saponins;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(6):1179-1182
- CountryChina
- Language:Chinese
-
Abstract:
AIM To develop an HPLC method for the simultaneous content determination of ginsenosides Rb1,Rd,F4,Rg1,20 (R)-Rg3,20 (S)-Rg3,Rgs,20 (R)-Rh1,20 (S)-Rh1,Rh4,Rk1,Rk3 and notoginsenoside R1 in Shusanqi Powder (processed Notoginseng Radix et Rhizoma).METHODS The analysis of 70％ methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Zorabax-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 203 nm.RESULTS Thirteen saponins showed good linear relationships within their own ranges (r =0.999 5),whose average recoveries were 90.01％-108.32％ with the RSDs of 0.62％-3.54％.CONCLUSION This sensitive and accurate method can be used for the quality control of Shusanqi Powder.
