Simultaneous determination of five constituents in Chairen Capsules by HPLC
10.3969/j.issn.1001-1528.2017.06.017
- VernacularTitle:HPLC法同时测定柴仁胶囊中5种成分
- Author:
Xuerong ZHANG
;
Chunqin MAO
;
Xue CHENG
;
Ji ZHANG
;
Lianlin SU
;
Tulin LU
- Keywords:
Chairen Capsules;
spinosin;
ferulic acid;
cinnamaldehyde;
liquiritin;
ammonium glycyrrhizate;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(6):1191-1194
- CountryChina
- Language:Chinese
-
Abstract:
AIM To develop an HPLC method for the simultaneous content determination of five constituents in Chairen Capsules (Bupleuri Radix,Ziziphi spinosae Semen,Chuanxiong Rhizoma,etc.).METHODS The analysis of 80％ methanol extract of this drug was performed on a 30 ℃ thermostatic Merck Purospher STAR C1s column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,290 and 335 nm.RESULTS Spinosin,ferulic acid,cinnamaldehyde,liquiritin and ammonium glycyrrhizate showed good linear relationships within the ranges of 0.009 26-0.092 6 μg (R2 =0.999 6),0.056 2-0.562 μg (R2 =0.999 8),0.130-1.30 μg (R2 =0.999 8),0.275-2.75 μg (R2 =0.999 5) and 0.568-5.68 μg (R2 =0.999 2),whose average recoveries were 100.73％,100.62％,100.02％,100.41％ and 99.96％ ％ with the RSDs of 2.20％,1.37％,1.52％,1.30％ and 0.80％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Chairen Capsules.
