Effect of kinase PLK4 on the proliferation of glioma cells in vitro

VernacularTitle:蛋白激酶PLK4对人脑胶质瘤增殖的影响
Author: Zuoxin ZHANG ; Junhu ZHOU ; Lei HAN
Keywords: glioma; kinase; PLK4; proliferation
From: Chinese Journal of Clinical Oncology 2017;44(14):685-688
CountryChina
Language:Chinese
Abstract: Objective:To analyze the correlation of protein kinase PLK4 with the prognosis of patients with malignant glioma and the proliferation of human glioblastoma cells. Methods:The relationship of PLK4 expression to the pathological grade and prognosis was evaluated using CGGA datasets. Designated siRNAs targeting PLK4 were constructed, and the silencing effect was identified by RT-qP-CR and Western blot analyses. The effect of PLK4 on the proliferation of tumor cells was evaluated by MTT and colony formation as-says. Results:The expression of PLK4 increased with pathological grade and was significantly related to the overall survival in patients with glioma and the prognosis of patients with high-grade glioma. MTT and colony formation assay results indicated that the prolifera-tion activity was significantly reduced after depleting PLK4. Conclusion:The expression of PLK4 is related to the prognosis of patients with glioma and can affect the proliferation activity of tumor cells. Therefore, PLK4 could be a potential therapeutic target in glioma treatment.