Expression and significance of TMSG-1 in esophageal squamous carcinoma and EC109 cells
10.7652/jdyxb201704013
- VernacularTitle:肿瘤转移抑制基因-1在食管鳞癌组织及食管癌EC109细胞中的表达及意义
- Author:
Bo LIU
;
Yang Lü
;
Yuanyuan LIN
;
Junchao LIU
;
Xiujuan LI
;
Haixia QIAO
- Keywords:
esophageal squamous cell carcinoma;
tumor metastasis suppressor gene-1 (TMSG-1);
EC109 cell;
immunohistochemistry;
RT-PCR;
cell proliferation;
cisplatin (CDDP)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(4):536-539,548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and significance of tumor metastasis suppressor gene-1(TMSG1) in esophageal squamous cell carcinoma (ESCC) and EC109 cells.Methods Immunohistochemistry S-P method was used to examine the expression of TMSG-1 protein in 136 cases of ESCC and 37 cases of normal esophageal mucosa.We analyzed the relationship between TMSG-1 and clinicopathological data of ESCC patients.EC109 cells were treated with 3 μg/mL of cisplatin (CDDP) in vitro for 24 h (the intervention group) and the control group was set up at the same time.The proliferation-inhibitory capability was analyzed with MTT assay.RT-PCR was used to examine the expression of TMSG-1 in the intervention group and the control group.Results The positive rate of TMSG-1 in ESCC and normal esophageal mucosa was 52.2％ (71/136) and 94.6％ (35/37),respectively.The expression of TMSG-1 in ESCC was significantly lower than that in normal esophageal mucosa (P＜0.05).The expression of TMSG-1 was related to TNM stage,differentiation degree and lymph node metastasis (P＜0.05).After EC 109 cells were treated with CDDP for 24 h,the proliferation inhibition rate was increased significantly compared with the control group (P＜0.01).RT-PCR results showed that the expression of TMSG-1 in the cells of the intervention group was significantly higher than that in the control group (P＜ 0.01).Conclusion The abnormal expression of TMSG-1 may play a role in the development and metastasis of ESCC.Examination of TMSG-1 may be useful for making diagnosis and guiding clinical therapy of ESCC.
