Effects of miR-34a on proliferation, migration and invasion of colon cancer SW480 cell and its possible mechanism
10.11904/j.issn.1002-3070.2017.03.001
- VernacularTitle:miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨
- Author:
Xiaojun HAO
;
Chuanzhuo WANG
;
Xiangxuan ZHAO
;
He XIN
;
Zhaoyu LIU
- Keywords:
Colorectal cancer;
miR-34a;
EMT;
Proliferation;
Invasion
- From:
Practical Oncology Journal
2017;31(3):193-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate effects of miR-34a on the proliferation,invasion and migration of colon cancer SW480 cell and its possible mechanism.Methods miR-34a overexpressed lentivirus and empty virus vector were transfected into SW480 cells and untreated cells were used as blank control group.Real-time PCR was used to detect the expression of miR-34a in each group.The cell proliferation was detected by CCK8 assay.The cell migration and invasion ability were detected by wound healing and transwell assays.The expression of E-cadherin and Vimentin protein was detected by Western blotting.Results Compared with the empty virus vector group and the blank control group,the expression of miR-34a was increased in the transfected cells,and the cell proliferation efficiency,invasion and migration ability were decreased in the transfected cells (P ＜ 0.05).miR-34a significantly increased the expression of E-cadherin protein and decreased Vimentin protein expression in the transfected cells.Conclusion miR-34a can inhibit the proliferation,invasion and migration of colon cancer SW480 cells,and affect the expression of E-cadherin and Vimentin.MR-34a is expected to be a potential molecular target for the metastasis and recurrence of colorectal cancer.
