Content Determination of Saccharide in Polysaccharides Containing Galacturonic Acid by Phenol-sulfuric Acid Method Combined with Calibration Factor Method
10.6039/j.issn.1001-0408.2017.21.26
- VernacularTitle:苯酚-硫酸法结合校正因子法测定含半乳糖醛酸的多糖中的糖含量
- Author:
Ruibin BAI
;
Yuling MA
;
Pei ZHANG
;
Yanping WANG
;
Yingdong LI
;
Fangdi HU
- Keywords:
Polysaccharides;
Galacturonic acid;
Codonopsis pilosula polysaccharides CPP1b;
Phenol-sulfuric acid method;
Correction factor method;
Saccharide content;
Determination
- From:
China Pharmacy
2017;28(21):2974-2978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of saccharide in polysaccharides containing galact-uronic acid based on phenol-sulfuric acid method combined with correction factor method. METHODS:The determination condi-tion of phenol-sulfuric acid was optimized. A series of standard curves were drawn with glacturonic aid-glucose mixed control with different mass ratio. The content of saccharide in Codonopsis pilosula polysaccharides CPP1b samples containing galacturonic acid was determined. According to regression equation of galacturonic acid-glucose ratio of 0-100%,the correction factor was calculated by using C. pilosula polysaccharides CPP1b as the reference polysaccharide,and the results of content determination of saccharide were corrected. The rationality of this method was verified by using mixed monosaccharide control with same composition as C. pi-losula polysaccharides CPP1b. RESULTS:The correction factor of C. pilosula polysaccharide CPP1b to glucose was 3.33;in vali-dation test,the content of saccharide in mixed monosaccharide control with same composition as C. pilosula polysaccharides CPP1b was 103.47%. RSDs of precision and stability tests was <1%. The recoveries ranged 93.52%-107.35%(RSD=5.09%,n=6). CONCLUSIONS:The established method can accurately determine the content of saccharide in C. pilosula polysaccharides CPP1b containing galacturonic acid.
