Study on Quality Standard of Zhuang Medicine Homalocladium platycladum
10.6039/j.issn.1001-0408.2017.21.30
- VernacularTitle:壮药竹节蓼的质量标准研究
- Author:
Xiangyan ZENG
;
Yurong TANG
;
Pinghua YAN
;
Li LI
- Keywords:
Zhuang medicine;
Homalocladium platycladum;
Quercetrin;
Qualitative;
Quantitative;
TLC;
HPLC
- From:
China Pharmacy
2017;28(21):2989-2991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish quality standard for Zhuang medicine Homalocladium platycladum. METHODS:TLC method was used for qualitative identification. HPLC method was used to determine the content of quercetrin in samples:Agilent Zorbax SB-C18 column,mobile phase consisted of acetonitrile-0.1% acetic acid(20:80,V/V),flow rate of 1.0 mL/min,detection wavelength of 360 nm,column temperature of 30 ℃,injection volume of 10 μL. The contents of moisture,total ash and extract in samples were determined. referring to Chinese Pharmacopiea(2015 edition). RESULTS:TLC spots of H. platycladum were clear and well-separated without interference from negative control. The linear range of quercetin were 0.023-0.46 mg/mL(r=0.9991). RSDs of precision,stability and repeatability tests were all lower than 2.0%. The average recoveries were 98.01%-103.05%(RSD=1.71%,n=6). The contents of moisture,total ash,extract and quercetrin in samples were 6.39%-9.78%,2.82%-8.07%, 0.02%-0.27%,14.14%-28.45%,0.09%-0.50% respectively. CONCLUSIONS:Established trial can be used for quality control of Zhuang medicine H. platycladum.
