Research Progress in Analysis of Small Molecule Metabolites inBio-matrices by Stable Isotope Coded Derivatization Combiningwith Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.170113
- VernacularTitle:稳定同位素标记衍生化结合液相色谱-串联质谱在生物样本中小分子代谢物分析的研究进展
- Author:
Yunlu HE
;
Yanbo LUO
;
Huan CHEN
;
Hongwei HOU
;
Qingyuan HU
- Keywords:
Isotope labeling;
Derivatization;
Liquid chromatography-tandem mass spectrometry;
Metabolite;
Biological samples;
Review
- From:
Chinese Journal of Analytical Chemistry
2017;45(7):1066-1077
- CountryChina
- Language:Chinese
-
Abstract:
Stable isotope coded derivatization (ICD) is an isotope labeling technique for specific functional groups of the target analytes through chemical derivatization.ICD combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables systematically analyzing the biomolecules with the same labeling reaction characteristic.ICD technique can effectively solve the limited sensitivity in complex bio-matrices analysis and unavailability of isotope internal standards in quantitative analysis.In recent years, ICD technique has been widely used in metabolomics research field.Based on this background, the ICD technique and the design of ICD reagents are briefly described in this review.The recent advances in ICD reagents for carboxyl, amino, carbonyl, thiol and hydroxyl groups and their applications in the analysis of small molecule metabolites in bio-matrices with LC-MS/MS are reviewed.
