Optimization of Extraction Technology of Panax Notoginseng and Scrophulariae Radix from Rupixiao Granule by Orthogonal Design
- VernacularTitle:正交试验优选乳癖消颗粒中三七和玄参的提取工艺
- Author:
Hongyu JI
;
Yuyang SUN
;
Bing SHAO
;
Bo CHEN
;
Linhua WU
- Keywords:
Rupixiao granule;
Orthogonal test;
Ginsenoside Rg1;
Ginsenoside Rb1;
Harpagoside
- From:
China Pharmacist
2017;20(7):1313-1316
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the extraction technology of Panax notoginseng and Scrophulariae radix from Rupixiao granule.Methods: With the dry extract rate and transfer rates of ginsenoside Rg1, ginsenoside Rb1 and harpagoside as the comprehensive index, the orthogonal design was adopted to investigate the effects of the amount and concentration of ethanol, extracting duration and times on the extraction technology.The contents of ginsenoside Rg1, ginsenoside Rb1 and harpagoside were determined by HPLC.Results: The optimal extraction technology was extracted twice with 8-fold amount of 60% ethanol with 2 h per time.The transfer rate of ginsenoside Rg1, ginsenoside Rb1 and harpagoside was (79.4%±1.56%), (42.62%±0.68%) and (44.89%±0.58%)(n=3), respectively.The dry extract rate was (20.99%±0.411%).Conclusion: The optimized extraction technology is stable and feasible, which can be used for extracting Panax notoginseng and Scrophulariae radix from Rupixiao granule.
