Validation of Microbial Limit Test for Compound Benzocaine Gel
- VernacularTitle:复方苯佐卡因凝胶微生物限度检查方法的验证
- Author:
Cuihong ZHAO
;
Buming LIU
;
Rui LI
;
Qiaoyan LU
;
Lianjiao YAN
- Keywords:
Compound benzocaine gel;
Microbial limit test;
Polysorbate 80;
Lecithin;
Neutralization method
- From:
China Pharmacist
2017;20(7):1328-1331
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a microbial limit test method for compound benzocaine gel.Methods: According to the general principles of Chinese Pharmacopoeia (2015 edition), method applicable experiments were performed respectively for the routine method, neutralization method and dilution & neutralization method.Using the recovery ratio of test bacteria as the index, the medium of dilution & neutralization method was adopted in the total aerobic microbial count and total combined yeasts and molds count, and the neutralization method was used for the control bacteria detection.The neutralizing agents were polysorbate 80 and lecithin.Results: The method of dilution & neutralization could eliminate the bacteriostasis of the drug, and the recovery ratio of each test bacteria was within the range of 0.5-2.0, which was in line with the requirements of Chinese Pharmacopoeia.Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus could be detected out in the positive control bacterial test,and bacteria were not detected out in the negative test.Conclusion: The method can be used for the microbial limit test for compound benzocaine gel.
