HPLC method for quantitative determination of Ginsenoside Rb1 in Jupizhuru decoction
10.13220/j.cnki.jipr.2017.06.034
- VernacularTitle:橘皮竹茹汤中人参皂苷Rb1的HPLC含量测定方法
- Author:
Zefeng CAO
;
Song GAO
;
Lei SUN
;
Shanyi QIAO
- Keywords:
quantitative analysis;
ginsenoside Rb1;
HPLC
- From:
Journal of International Pharmaceutical Research
2017;44(6):647-650
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for the determination of ginsenoside Rb1 in Jupizhuru decoction,and de-termine the amount of ginsenoside Rb1 in 10 batches of Jupizhuru decoction composed of different batch herbs. Methods The HPLC analysis was carried out on a Phenomenex Luna C18 column with column temperature at 30℃,flow rate 1.0 ml/min,detection wave-length 203 nm and mobile phase acetonitrile and 0.1%phosphoric acid by gradient elution. Results The method was found to be lin-ear within the ranges of 5-500μg/ml(r=0.9999),and the average recovery of ginsenoside Rb1 was 95.5%(RSD 1.77%,n=6). Con-clusion The determination method is suitable for the quality control of ginsenoside Rb1 in Jupizhuru decoction.
