Establishment of fingerprint profiles of polysaccharides extracted from Guifu Dihuang Wan
10.13220/j.cnki.jipr.2017.06.032
- VernacularTitle:桂附地黄丸中多糖指纹图谱的建立
- Author:
Haitao FAN
;
Lei SUN
;
Shanyi QIAO
- Keywords:
polysaccharides;
Guifu Dihuang Wan;
partial hydrolysis;
fingerprint profile
- From:
Journal of International Pharmaceutical Research
2017;44(6):638-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find a new method for quality control of polysaccharides by establishing the fingerprint profiles of polysaccharides extracted from Guifu Dihuang Wan. Methods The polysaccharides extracted from Guifu Dihuang Wan were hydro-lyzed partially under appropriate hydrolysis condition with acid,and the fingerprint profiles of the hydrolyzates was obtained by HPLC. Microsoft Excel 2002 was applied for calculating the similarity of fingerprint profiles,and informatton analysis of these profiles was performed by a software for evaluating fingerprint profiles of traditional Chinese medicine. Results Fingerprint profiles of the hydroly-zates of polysaccharides from 10 of Guifu Dihuang Wan products,produced by different manufacturers,were obtained. It was observed that the similarity of eight products was greater than 0.99,while the similarity of the other two was 0.92 and 0.94,respectively. Con-clusion This method is technically feasible for the quality control of polysaccharides from Guifu Dihuang Wan.
