Effect of Serum on Cellular Uptake of TetrahedralDeoxyribonucleic Acid Nanostructure
10.11895/j.issn.0253-3820.170104
- VernacularTitle:血清对细胞摄取DNA四面体纳米结构行为的影响
- Author:
Xingjie HU
;
Zejun WANG
;
Min YIN
;
Di LI
;
Nan CHEN
- Keywords:
Deoxyribonucleic acid nanostructure;
Deoxyribonucleic acid tetrahedron;
Cellular uptake;
Serum;
Endocytosis
- From:
Chinese Journal of Analytical Chemistry
2017;45(7):951-957
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, the effect of serum (fetal bovine serum, FBS) on cellular uptake behaviors of DNA tetrahedron (TDNs) was investigated.Fluorescence-labeled TDNs were synthesized by self-assembly and purified with HPLC to achieve TDNs product with purity of 95%.By means of flow cytometry and confocal microscopy, the kinetics of TDNs endocytosis in HeLa cells in the presence or absence of FBS was compared.The results showed that TDNs remained intact in complete medium and cell lysate for more than 12 hours.FBS increased the amount of internalized TDNs in HeLa cells, whereas it did not change the route of caveolin-dependent endocytosis.Herein, our study provided a new insight into the effects of biomolecules on the interaction between cells and DNA nanostructures, which helped the future development of DNA-based intracellular nanocarriers.
